World's smallest AO fluorescence microscope using DPP technology


In an article in Optics Express, we describe a simple practical application of our Deformable Phase Plate: A miniaturized fluorescence microscope, which can even be considered the "World's Smallest AO Microscope".


In this work we have combined our refractive Ultra-Thin Deformable Phase Plate with a Sensorless Wavefront Aberration Estimation algorithm and demonstrate diffraction-limited imaging of synthesized samples in a fully refractive AO microscope. Replacement of the conventional deformable mirrors with our DPP results in avoiding beam-folding and complex system re-design and yet, conserves the high order aberration correction capability.


You can read the open access paper here:


Original article: "Fully refractive adaptive optics fluorescence microscope using an optofluidic wavefront modulator" https://doi.org/10.1364/OE.387734

#microscopy #fluorescent #nikon #worldrecord #refractiveAO

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