Together with Prof. Alexander Jesacher’s group at the Institute for Biomedical Physics, Medical University of Innsbruck, and the Microsystems for Biomedical Imaging Laboratory at IMTEK, University of Freiburg, we have published “Optofluidic adaptive optics in multi-photon microscopy” in Biomedical Optics Express.

Sensorless correction, adapted to a transmissive modulator

The paper introduces a fast and robust sensorless adaptive optics scheme written for transmissive wavefront modulators: the aberration is estimated from the images themselves, and the correction is applied through the Deformable Phase Plate — transmissive, refractive, polarization-independent and broadband. In simulations and experiments it demonstrates scatter correction of two-photon-excited fluorescence images of microbeads and of brain cells, and benchmarks the DPP against a liquid-crystal spatial light modulator.

That opens adaptive optics to setups where the restriction to reflective or diffractive correctors had held it back. The paper is open access.

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